Dr. Guolu Zheng
211 Foster Hall, Lincoln University
820 Chestnut Street, Jefferson City, Missouri 65101
Phone: 573-681-5964; email: ZhengG@LincolnU.edu
1998-2000 Postdoctoral Fellow, Department of Biochemistry and Molecular Biology, Oregon Graduate Institute of Science and Technology, Beaverton, Oregon. (Now it is a part of Oregon Health and Science University)
1993-1997 Ph.D. University of Arkansas, Fayetteville, Arkansas
1986-1989 M.S. Xiamen University, P. R. China
1979-1983 B.S. Xiamen University, P. R. China
2014-present Associate Professor, Cooperative Research Programs, Lincoln University, Jefferson City, Missouri
2009-present Principal Investigator, Cooperative Research Programs, Lincoln University, Jefferson City, Missouri
2010-present Adjunct Assistant Professor, Division of Food Science and Bioengineering, University of Missouri, Columbia, Missouri
2009-2014 Assistant Professor, Cooperative Research Programs, Lincoln University, Jefferson City, Missouri
2006-2009 Research Assistant Professor, Department of Veterinary Biopathology, University of Missouri-Columbia, Columbia, Missouri
2003-2005 Research Assistant Professor, University of Rochester School of Medicine and Dentistry, Rochester, New York
2000-2003 Senior Research Associate, Department of Biochemistry and Molecular biology, Oregon Graduate Institute of Science & Technology, Beaverton, Oregon
2009-present Member, Human Subjects and Recombinant DNA Review Committee
Member, Environmental Stewardship Committee
- American Society for Microbiology, contributing member
- International Association for Food Protection, member
- Microbial safety of food and water
- Microbial source tracking
Title: Use of Endophytic Microorganisms to Improve the Food Safety of Fresh and Fresh-cut Produce
The number of foodborne disease outbreaks associated with fresh and fresh-cut produce has increased in recent years in the United States. However, current postharvest washing and sanitization processes do not always effectively reduce pathogen contamination in/on produce, and there is increasing concern about the chemical compounds used in postharvest sanitation. Therefore, novel control methods are critically needed. This proof-of-concept project tackles the use of endophytic microorganisms for the competitive exclusion of potential human pathogens on fresh produce. Endophytic microbes are symbiotic and able to live inside plants from farm to table. We hypothesize that produce naturally carrying single or a mixture of anti-human-pathogen endophytes can safeguard in situ fresh and fresh-cut produce against potential human pathogens from farm to table. Romaine lettuce and human pathogen E. coli O157:H7 (EcO157) were selected for this study to prove the concept of the in-situ biocontrol of human pathogens in fresh and fresh-cut produce. We will first to isolate abti-EcO157 from the internal tissues of lettuce and then evaluate their efficiencies on controlling EcO157 in lettuce pre- and post- harvest. Success of this project can lead to developing an environmentally sound, user-friendly, economic, and efficient biocontrol technique for farmers of produce at local, state, and national level.
- AGR 595 Scientific Writing
- AGR 555 Food Safety: Theory and Practice
PEER-REVIEWED PAPERS in the last 5 years (* corresponding author)
- Yuan, Y., Zheng, G., Lin, M, Mustapha, A. 2018. Detection of viable Escherichia coli in environmental water using combined propidium monoazide staining and quantitative PCR. Water Res. 15 (145):398-407.
- Zheng G.,* Shen, Z. 2018. Host-specific genetic markers of fecal bacteria for fecal source tracking in food and water. J Food Microbiol Saf Hyg 3: 135. Doi: 10.4172/2476-2059.1000135
- Shen, Z., Mustapha, A., Lin. M., Zheng, G*. 2017. Biocontrol of the internalization of Salmonella enterica and Enterohaemorrhagic Escherichia coli in mung bean sprouts with an endophytic Bacillus subtilis. Int J Food Microbiol. 24(250):37-44.
- He, X., Liu, P., Zheng, G., Chen, H., Shi, W., Cui, Y., Ren, H., Zhang, X.X. 2016. Evaluation of five microbial and four mitochondrial DNA markers for tracking human and pig fecal pollution in freshwater. Sci Rep. 13(6) 6:35311
- Shen, Z., Zhang, N., Mustapha, A., Lin, M., Xu, D., Deng, D., Reed, M., Zheng, G* 2016. Identification of host-specific genetic markers within 16S rDNA intervening sequences of 73 genera of fecal bacteria. J Data Mining Genomics & Proteomics 7:1 http://dx.doi.org/10.4172/2153-0602.1000186
- Deng, D., Liu, J., Xu, M., Zheng, G., Guo, J., Sun, G. 2016. Uptake, translocation andmetabolism of decabromodiphenyl ether (BDE-209) in seven aquatic plants. Chemosphere. 152:360-8.
- Deng, D., N. Zhang, D. Xu, M. Reed, G. Zheng*. 2015. Polymorphism of the glucosyltransferase gene (ycjM) in Escherichia coli and its use for tracking human fecal pollution in water. Sci Total Environ. 15; 537:260-7.
- Deng, D., N. Zhang, A. Mustapha, D. Xu, T. Wuliji, M. Farley, J. Yang, B. Hua, F. Liu, G. Zheng.* 2014. Differentiating enteric Escherichia coli from environmental bacteria through the putative glucosyltransferase gene (ycjM). Water Res. 61: 224-31.
- US Patent 5,821,546: Xiao, Min; Zhuang, Da Kui; Zheng, Guolu; Slavik, Michael F; Method and system for fecal detection
- Lincoln University of Missouri Invention Disclosure: Zheng, Guolu., Dong Xu, and Chao Zhang. 2013: Identification of host-specific DNA sequences of microorganisms using combinatorial entropy of DNA sequences.
- Zheng, G. 2019. E. coli, or not E. coli, that is the question. ARD Updates. 10(2): 1-2.